Quality of the calcium-enriched mixture apical plug in simulated apexification model; Effect of different mixing and placement methods

Background Presence of voids at root canal wall‒apical seal material interface gives rise to the entrapment of toxins and microorganisms, which might have a relationship with post treatment disease. The present study was carried out to evaluate the effect of different mixing (manual and ultrasonic) and placement (manual and manual in association with indirect ultrasonic) methods of Calcium-enriched Mixture (CEM) cement on the number and dimensions of voids in the apical plug in simulated apexification models. Material and Methods A total of 80 human maxillary central incisors with mature apices were selected. After simulation of the open apices, the teeth were divided into 4 groups (n=20) based on the mixing and placement techniques of CEM cement: group 1, manual mixing‒manual placement; group 2, manual mixing‒manual placement in association with indirect ultrasonic technique; group 3, ultrasonic mixing‒manual placement; and group 4, ultrasonic mixing‒manual placement in association with indirect ultrasonic technique. The samples were placed within gypsum sockets in which the periodontal ligament was reconstructed with polyether impression material. After placement the apical plugs, a wet piece of cotton was placed on canal orifices, followed by dressing with Cavit. The samples were incubated at 37°C and 100% relative humidity for 7 days. Then the voids between the material and root canal walls were counted with the CBCT technique. The void dimensions were scored with the following scoring system: score 1, no voids; score 2, the void size less than half of the size of the evaluated cross-section; score 3, the void size larger than half of the size of the evaluated cross-section. Statistical analyses were carried out with chi-squared and Fisher's exact tests. Statistical significance was defined at P<0.05. Results The maximum (7) and minimum (2) number of voids were detected in groups 1 and 2, respectively. The difference between these two groups was statistically significant (p<0.05). The differences in the number of voids between groups with similar mixing technique and different mixing techniques (i.e. groups 1 and 3 and groups 2 and 4) were not significant (p>0.05). Void dimensions in all the study groups were in score 2 category and no score 3 was recorded in the study groups. Conclusions Under the limitations of the present study, manual placement in association with indirect ultrasonic technique was a proper technique to improve the quality of apical plug, considering the decrease in the number of voids. Key words:Apical plug, CEM cement, void.